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KMID : 0364820130490010017
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Korean Journal of Microbiology
2013 Volume.49 No. 1 p.17 ~ p.23
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Inhibition of Candida albicans Biofilm Formation by Coptidis chinensis through Damaging the Integrity of Cell Membrane
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Kim Youn-Hee
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Abstract
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Candida biofilms are organized microbial communities growing on the surfaces of host tissues or indwelling
medical devices, and the biofilms show enhanced resistance against the conventional antifungal agents. The roots of
Coptidis chinensis have been widely used for medicinal purposes in East Asia. The present study was aimed to
assess the effect of C. chinensis aqueous extract upon preformed biofilms of 10 clinical Candida albicans isolates
and the antifungal activities which contribute to inhibit the C. albicans biofilm formation. Its effect on preformed
biofilms was judged using XTT [2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)]
reduction assay, and metabolic activity of all tested strains was reduced significantly (57.3 ± 14.7%) at 98 μg/ml of
the C. chinensis extract. The extract damaged the cell membrane of C. albicans which was analyzed by fluorescein
diacetate and propidium iodide staining. The anticandidal activity was fungicidal, and the extract obstructed the
adhesion of C. albicans biofilms to polystyrene surfaces, arrested C. albicans cells at Go/G1 as well, and reduced the
growth of biofilms or budding yeasts finally. The data suggest that C. chinensis has multiple antifungal effects on
target fungi resulting in preventing the formation of biofilms. Therefore, C. chinensis holds great promise for
exploring antifungal agents from natural products in treating and eliminating biofilm-associated Candida infection.
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KEYWORD
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Candida albicans, Coptidis chinensis, antifungal activity, biofilm, cell membrane
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